Identification and characterization of synovial mesenchymal stem cells in temporomandibular joint]
[Article in Chinese]
Li J,
Long X,
Ke J,
Meng QG,
Fang W.
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
OBJECTIVE: To identify and characterize mesenchymal stem cells from synovial membrane of temporomandibular joint in vitro. METHODS: Synovial mesenchymal stem
cells (SMSCs) were obtained by limited dilution method and expanded in 25 ml flasks. Methyl thiazolyl tetrazolium (MTT) method was used to determine the cell
growth cycles. The expressions of vimentin and keratin were respectively detected with immunocytochemistry, while the expressions of CD8, CD34, CD44, vascular
cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were determined by flow cytometry. RESULTS: Pure mesenchymal stem cells were
of spindle shape and uniform in size, which were intensively positive in vimentin, but negative in keratin. The expression of CD44, VCAM-1 and ICAM-1 were also
verified by flow cytometry. CONCLUSIONS: Mesenchymal stem cells could be purified from adult synovial membrane of temporomandibular joint.
Publication Types:
PMID: 16255911 [PubMed - in process]
http://www.ncbi.nlm.nih.g...ed/16255911?dopt=Abstract
